[Analysis of tissue bioavailability of total polyphenols by Folin Ciocalteu and Fast Blue BB techniques in organs of BALB/C/c mice].

Biomedical potential of polyphenols lies in their ability to modulate redox balance and the mechanisms involved in the development of chronic noncommunicable diseases.

OBJECTIVE: The aim of this study was to determine the concentration of total polyphenols in different murine organs by assaying analytical techniques of Folin Ciocalteu (FC) and Fast Blue BB (FBBB).

METHOD: Balb/c female mice (n≥3) received for 15 days 100 mg/kg/d of extract of Lantana grisebachii (LG), Aspidosperma quebracho-blanco (AQB) or Ilex paraguariensis (IP) and control group (treated with water without extract). Polyphenolic concentrations were measured in telencephalon, diencephalon, midbrain, brainstem, cerebellum, spleen, thymus and cardiopulmonary tissue by FC and FBBB methods. Results were compared by ANOVA followed by Tukey test (p<0.05).

RESULTS: FBBB method reported higher detections than FC (4.5 fold in telencephalon, 8.4 in midbrain, 5 in brainstem, 7.2 in spleen, 68.5 in thymus and 4 in cardiopulmonary tissue). Regarding the treatments, the group that received AQB showed to have increased polyphenolic bioavailability in brainstem (p<0.02). With FBBB, a decrease on thymic polyphenol content after treatment with IP was detected (p<0.005). In cerebellum of the groups treated with IP and telencephalon of the control group showed significant differences when these were analyzed with FC (p<0.05, p<0.0035 respectively).

CONCLUSIONS: FBBB method showed higher estimations of polyphenolic bioavailability than FC, and this could be related to higher specificity of the technique to react with phenolic compounds.