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[Effect of PDK1 on Neutrophil Differentiation].
Zhongguo Shi Yan Xue Ye Xue za Zhi 2017 June
OBJECTIVE: To knock out PDK1 by using Vav-Cre and observe the effects of PDK1 knock out on the ratio, number and differentiation of neutrophil.
METHODS: The PDK1 expression level of Vav-Cre;PDK1lox/lox mouse in the bone marrow cells was analyzed by RT-PCR. The effect of PDK1 on hematopoietic progenitor was observed by CFU-C assay, and the effect of PDK1 on the ratio and number of neutrophil was detected by flow cytometric analysis.
RESULTS: The RNA expression level of bone marrow cells of Vav-Cre;PDK1lox/lox mouse was dramatically lower than that of the control mouse. The number of functional GMP was lower in Vav-Cre;PDK1lox/lox mouse in contrast to controls. The percentage and number of neutrophil were lower, but the percentage of premyelocyte/myelocytes was more than 2 times in Vav-Cre;PDK1lox/lox group compared with control groups.
CONCLUSION: PDK1 affects the process of the differention of hematopoietic stem cells to GMP, the neutrophil differention and maturation.
METHODS: The PDK1 expression level of Vav-Cre;PDK1lox/lox mouse in the bone marrow cells was analyzed by RT-PCR. The effect of PDK1 on hematopoietic progenitor was observed by CFU-C assay, and the effect of PDK1 on the ratio and number of neutrophil was detected by flow cytometric analysis.
RESULTS: The RNA expression level of bone marrow cells of Vav-Cre;PDK1lox/lox mouse was dramatically lower than that of the control mouse. The number of functional GMP was lower in Vav-Cre;PDK1lox/lox mouse in contrast to controls. The percentage and number of neutrophil were lower, but the percentage of premyelocyte/myelocytes was more than 2 times in Vav-Cre;PDK1lox/lox group compared with control groups.
CONCLUSION: PDK1 affects the process of the differention of hematopoietic stem cells to GMP, the neutrophil differention and maturation.
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