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A composite polynomial approach for analyzing the indefinite self-association of macromolecules studied by sedimentation equilibrium.

Biophysical Chemistry 2017 September
A different approach is described for analyzing sedimentation equilibrium experiments of indefinitely self-associating systems. The procedure involves application of conservation of mass criteria, along with local evaluation of the weight average molar mass, to generate a polynomial based on a composite pseudo-independent variable. The outlined method does not depend upon non-linear regression to generate a solution, but instead requires evaluation of the roots of a high-order polynomial.

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