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[Protective effect of quercetin against seawater instillation-induced acute lung injury through preventing M1 polarization of macrophages in mice].

Objective To study the protective effect of quercetin against seawater instillation-induced acute lung injury (SW-ALI) and explore the roles of M1polarization of macrophages and M1 macrophage-mediated inflammation in the protective effect. Methods Male C57BL/6 mice were randomly divided into four groups, normal control group, quercetin-treated group, SW-ALI group, SW-ALI and quercetin-treated group, with 15 mice in each group. The mice were intraperitoneally injected with 1 g/L pentobarbital sodium, and then seawater was injected into the lungs through oropharyngeal intubation at the concentration of 4 mL/kg. Quercetin (200 mg/kg) was intraperitoneally injected 0.5 hour before seawater injection. The same amount of PBS and quercetin was intraperitoneally injected in normal control group and quercetin control group, respectively. Blood gas analysis of 1 mL blood taken from abdominal aorta was performed at 0.5, 1, 2, 3, 4 hours after different treatment. Lungs were obtained at 3 days after different treatment for frozen section preparation, HE staining, dry/wet ratio (D/W) analysis and the detection of inducible nitric oxide synthase (iNOS), interleukin 4 (IL-4), IL-10, IL-1β and tumor necrosis factor α (TNF-α) expressions by real-time quantitative PCR (qRT-PCR) and Western blotting. Results Quercetin significantly reduced the pathological changes of lung tissues, lowered PaCO2 while increased PaO2 and D/W ratio, down-regulated the expressions of iNOS, TNF-α, IL-1β while up-regulated the expressions of IL-4 and IL-10 in SW-ALI mice. Conclusion Quercetin can significantly improve the function of gas exchange and reduce the degree of lung injury in SW-ALI mice by inhibiting M1 polarization of macrophages and the expressions of pro-inflammatory factors.

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