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Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Activated Ion-Electron Transfer Dissociation Enables Comprehensive Top-Down Protein Fragmentation.
Journal of Proteome Research 2017 July 8
Here we report the first demonstration of near-complete sequence coverage of intact proteins using activated ion-electron transfer dissociation (AI-ETD), a method that leverages concurrent infrared photoactivation to enhance electron-driven dissociation. AI-ETD produces mainly c/z-type product ions and provides comprehensive (77-97%) protein sequence coverage, outperforming HCD, ETD, and EThcD for all proteins investigated. AI-ETD also maintains this performance across precursor ion charge states, mitigating charge-state dependence that limits traditional approaches.
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