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Neuron Segmentation With High-Level Biological Priors.
IEEE Transactions on Medical Imaging 2018 April
We present a novel approach to the problem of neuron segmentation in image volumes acquired by an electron microscopy. Existing methods, such as agglomerative or correlation clustering, rely solely on boundary evidence and have problems where such an evidence is lacking (e.g., incomplete staining) or ambiguous (e.g., co-located cell and mitochondria membranes). We investigate if these difficulties can be overcome by means of sparse region appearance cues that differentiate between pre- and postsynaptic neuron segments in mammalian neural tissue. We combine these cues with the traditional boundary evidence in the asymmetric multiway cut (AMWC) model, which simultaneously solves the partitioning and the semantic region labeling problems. We show that AMWC problems over superpixel graphs can be solved to global optimality with a cutting plane approach, and that the introduction of semantic class priors leads to significantly better segmentations.
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