[The Role of HMGN2 in the Development of Periodontitis Dental Plaque].

OBJECTIVES: To determine the role of high mobility group chromosomal protein N2 (HMGN2) in the development of periodontitis plaque biofilm.

METHODS: Saliva samples were collected before clinical interventions in patients with periodontitis (25 Mild periodontitis, 25 Moderate periodontitis and 25 Severe periodontitis) and healthy controls ( n =25).Following an estimation of dental plaque index, the level of HMGN2 in saliva was determined by enzyme-linked immunosorbent assay (ELISA).The recombinant human HMGN2 protein was purified and tested, its inhibitive effects on Porphyromonas gingivalis (P.g) growth and formation of P.g bioflim were detected by K-B antibacterial annulus and crystal violet staining, respectively.

RESULTS: The healthy controls had lower levels of HMGN2 in saliva than those with periodontitis, especially those with severe periodontitis ( P <0.01).The level of HMGN2 was negatively correlated with dental plaque index ( r =-0.363, P <0.05). HMGN2 inhibited the development of main periodontal bacteria biofilm ( P <0.05).

CONCLUSIONS: HMGN2 is involved in the development of periodontitis plaque biofilm and plays an important role in the development of periodontitis.