Multivitamins preventive therapy against subclinical endometritis in buffaloes: Its correlation to NEFA and oxidative stress.

The current study was designed to elucidate the in vivo antioxidant, preventive, and ameliorating effects of vitamins AD3 E on the incidence of subclinical endometritis (SCE) in buffaloes. Twenty-four buffaloes were divided equally into two groups; group I: control and group II: received AD3 E combination. Endometrial cytological samples (n=48) were collected using cytobrush to diagnose SCE by counting polymorphonuclear cells (PMN) ≥6% at 5th (W5) and≥4% at 7th (W7) weeks postpartum. Results revealed that serum superoxide dismutase and nitric oxide significantly increased and decreased, respectively at W7 in AD3 E group. The PMN% were significantly correlated with oxidative/anti-oxidative stress markers at W5 and W7. Vaginal score, PMN%, and blood neutrophils were significantly higher in the control group buffaloes than the AD3 E enriched ones. Therefore, the prevalence of SCE reduced significantly in the AD3 E supplemented buffaloes as compared to the control ones at W5 (23.15% and 38.46%) and W7 (9.8% and 32.34%), respectively. The control group revealed higher NEFA levels (P≤0.05) at W5 and W7 than the AD3 E group. The AD3 E supplemented buffaloes had shorter days open and higher pregnancy rate at 120th and 150th days postpartum than the control ones. In conclusion, micronutrients (AD3 E) intervention acts as a safeguard against the incidence of postpartum SCE and significantly improves the reproductive performance of buffaloes.