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JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
Terbium(III) Luminescent Complexes as Millisecond-Scale Viscosity Probes for Lifetime Imaging.
Journal of the American Chemical Society 2017 June 15
Fluorescent probes that are able to directly measure viscosity are attractive candidates for the study of intracellular environments. We report a new class of luminescent rotors, based on the sensitized emission of a terbium(III) complex. A 4-fold increase in both quantum yield and luminescence lifetime was observed in viscous media for the studied complexes, with a lifetime ranging from 0.23 to 0.89 ms over a broad range of viscosities (0.6-1200 cP). The presented approach, relying on the millisecond-scale luminescence lifetime of the lanthanide ions, was applied to fixed T24 cancer cells using temporal sampling lifetime imaging microscopy.
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