Add like
Add dislike
Add to saved papers

A novel multi-purpose enzymatic system and procedures for the rapid fluorescent determination of flavonoids in herbal pharmaceuticals and plant materials.

Talanta 2017 August 16
The paper presents a novel multi-purpose enzymatic system and procedures for fluorescent determination of several flavonoids in herbal pharmaceuticals and plant materials after their enzyme-catalyzed oxidation by hydrogen peroxide and further derivatization with meso-1,2-diphenylethylenediamine. This system may be used for rapid (15-30min/20 samples) simultaneous screening of samples containing a certain flavonoid in a standard microplate, or as a HPLC detection system for analyzing plant extracts with uncertain composition. In the first case, this indicator system provides sensitive and reproducible microplate determination of quercetin, epicatechin, caffeic acid, and taxifolin in the ranges 0.1-5, 1-10, 0.1-10, 0.5-5μM, respectively. In the second case, quercetin, epicatechin, and caffeic acid can be determined in the ranges 0.05-0.75, 0.05-0.75, and 0.01-0.75µg/ml (0.16-2.5, 0.17-2.6, 0.06-4.2μM), respectively. We have demonstrated the application of the system for the analysis of 3 pharmaceuticals and 3 types of plants.

Full text links

We have located links that may give you full text access.
Can't access the paper?
Try logging in through your university/institutional subscription. For a smoother one-click institutional access experience, please use our mobile app.

For the best experience, use the Read mobile app

Mobile app image

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app

All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.

By using this service, you agree to our terms of use and privacy policy.

Your Privacy Choices Toggle icon

You can now claim free CME credits for this literature searchClaim now

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app