Codonopsis lanceolata polysaccharide CLPS inhibits melanoma metastasis via regulating integrin signaling.

β1 integrin-mediated migration plays a fundamental role in melanoma metastasis. Here, we evaluated the molecular mechanisms underlying the anti-metastatic capacity of a polysaccharide fraction (CLPS) from Codonopsis lanceolata. CLPS inhibited in vivo melanoma metastasis in a B16F10 pulmonary metastasis model, impaired β1 integrin-mediated B16F10 melanoma cell migration in vitro evaluated by Transwell assay, reduced affinity between β1 integrin and ligand protein GST-FNIII9-10 . Also, CLPS attenuated the adhesion-dependent formation of focal adhesion and blocked β1 integrin/FAK/paxillin signaling axis. These findings illustrate a better understanding underlying the anti-metastatic activity of CLPS, indicating a potential treatment strategy for melanoma metastasis.