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Rodent Whole-Body Sectioning and MALDI Mass Spectrometry Imaging.
Here, we describe a method for obtaining whole-body MALDI imaging data. MALDI imaging provides chemical compound-specific information not attainable with conventional histology techniques. The specificity of mass spectrometry with the addition of spatial information makes this a very powerful technique, especially for the analysis of endogenous and exogenous small molecules. This chapter will provide the reader with a comprehensive description of the techniques involved in obtaining high-quality MALDI mass spectrometry imaging (MSI) data from large tissue sections.
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