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Re-characterization of hammerhead ribozymes as molecular tools for intermolecular RNA cleavage.
Organic & Biomolecular Chemistry 2017 May 32
A new guideline for the construction of hammerhead ribozymes to achieve trans-cleavage of a single-stranded RNA molecule was developed. The sequence rule of the HHRz cleavage site was highly recommended to be "DWH" with an optimal binding arm length of 8-9nt, which diverged from the former rule of "NUX".
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