Room Temperature X-Ray Crystallography Reveals Conformational Heterogeneity of Engineered Proteins.

In this issue of Structure, Biel et al. (2017) present multi-conformer analyses from room temperature X-ray data of two ubiquitin phage display variants binding deubiquitinase USP7. The first contains core mutations. The second matured variant contains additional surface mutations. Alternate conformations detected in the core mutant were removed by maturation.