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Pyrophosphate as substrate for alkaline phosphatase activity: A convenient flow-injection chemiluminescence assay.
A sensitive and convenient flow-injection chemiluminescence (FI-CL) turn-on assay for alkaline phosphatase (ALP) activity without any label and synthesis is developed. Cu2+ can catalyze the luminol-H2 O2 CL reaction. Pyrophosphate (PPi) can chelate Cu2+ and therefore the Cu2+ -mediated luminol-H2 O2 CL reaction is inhibited. The addition of ALP can catalyze the hydrolysis of PPi into phosphate ions, Cu2+ is released and the chemiluminescence recovers. A detection limit of 1 mU/mL ALP is obtained.
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