Strain specific variation in plant growth promoting volatile organic compound production by five different Pseudomonas spp. as confirmed by response of Vigna radiata seedlings.

AIM: Analysis of blend of volatile organic compounds (VOCs) released by Pseudomonas spp. with growth promoting effect in Vigna radiata seedlings.

METHODS AND RESULTS: Plant growth promoting activity of VOCs produced by five different Pseudomonas spp. was investigated by I-plate technique. VOCs produced by different organisms were extracted by various solvents (methanol, ethyl acetate, hexane and butanol) were identified by gas chromatography-mass spectrometric (GC-MS) analysis. The major VOCs identified were undecane, nonadecane, hexacosane, tetradecane, heptacosane, pentadecane, dodecane and tetratetracontane. The cultural conditions for the production of VOCs with plant growth enhancement effect were optimized and further confirmed using pure pentadecane as a candidate VOC.

CONCLUSIONS: The study provides insight into plant beneficial effect of VOCs produced by Pseudomonas spp. Remarkable modulation in production of VOCs with plant growth promoting effect by rhizobacteria was found to be depend on bacterial strain and its concentration. This article is protected by copyright. All rights reserved.