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Ratiometric Singlet Oxygen Detection in Water Using Acene-Doped Conjugated Polymer Nanoparticles.
ACS Applied Materials & Interfaces 2017 May 11
Although fluorescent probes for the detection of singlet oxygen (1 O2 ) have been an active area of research, most such probes rely upon change in intensity of a single band. Herein, we report a FRET-based, 1 O2 -sensitive aqueous suspension of conjugated polymer nanoparticles (CPNs) comprising the energy-donating host polymer poly[{9,9-dioctyl-2,7-divinylene-fluorenylene}-alt-co-{2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylene}] doped with an energy acceptor, the 1 O2 -sensitive thienoacene 5,12-bis(4-methoxyphenyl)-2-butyltetraceno[2,3-b]thiophene. Using a phthalocyanine-based sensitizer, IRDye 700DX, our probe shows a rapid, ratiometric response to photosensitized 1 O2 in water in both cuvettes and 96-well plates that compares favorably to the commercial 1 O2 -sensitive dye, singlet oxygen sensor green. The response to irradiation of even nanomolar concentrations of photosensitizer demonstrates the sensitivity of our ratiometric probe.
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