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Development of certified reference materials for accurate determination of fluoroquinolone antibiotics in chicken meat.
Food Chemistry 2017 August 16
Certified reference materials (CRMs; KRISS CRM 108-03-003, 108-03-004) were developed for the accurate determination of fluoroquinolones (enrofloxacin and ciprofloxacin, respectively) in chicken meat. Two groups of chickens were cured with feeds containing enrofloxacin and ciprofloxacin, respectively. After slaughter, the thigh and breast meats were combined for the respective groups and the meat was freeze-dried, pulverized, sieved, and V-mixed. The final bulk material was bottled in 10g portions. For certification of the CRMs, isotope dilution-liquid chromatography/tandem mass spectrometry was used. The certified values of the CRMs were (19.06±0.86)mg/kg for enrofloxacin and (1.095±0.038)mg/kg for ciprofloxacin. The stabilities of the CRMs were monitored at -70°C for 12months, at -20°C for 2months, and at room temperature for 1month. Both CRM candidates were stable during the monitoring period for each temperature.
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