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Surface-imprinted magnetic nanoparticles for the selective enrichment and fast separation of fluoroquinolones in human serum.
Journal of Separation Science 2017 May
Surface enrofloxacin-imprinted magnetic nanoparticles were prepared for the selective recognition and fast separation of fluoroquinolones in human serum by surface-initiated reversible addition fragmentation chain transfer polymerization. The surface morphology and imprinted behavior were investigated and optimized. The living/controlled nature of reversible addition-fragmentation chain transfer polymerization reaction allowed the successful construction of well-defined imprinted polymer layer outside the Fe3 O4 core. Such molecularly imprinted polymers exhibited superparamagnetic properties and specific recognition toward fluoroquinolones. Combined with reversed-phase high-performance liquid chromatography, the prepared molecularly imprinted polymers were used for the selective enrichment and analysis of fluoroquinolones in human serum samples. The recoveries of four fluoroquinolones were 86.8-95.3% with relative standard deviations of 2.0-6.8% (n = 3). Such magnetic molecularly imprinted polymers have great prospects in the separation and enrichment of trace analysts in complex biological samples.
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