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Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review
The CRISPR-Cas9 system in Neisseria spp.
Pathogens and Disease 2017 June 2
Bacteria and archaea possess numerous defense systems to combat viral infections and other mobile genetic elements. Uniquely among these, CRISPR-Cas (clustered, regularly interspaced short palindromic repeats-CRISPR associated) provides adaptive genetic interference against foreign nucleic acids. Here we review recent advances on the CRISPR-Cas9 system in Neisseria spp, with a focus on its biological functions in genetic transfer, its mechanistic features that establish new paradigms and its technological applications in eukaryotic genome engineering.
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