We have located links that may give you full text access.
Metal oxide surfaces for enhanced colorimetric response in bioassays.
Colloids and Surfaces. B, Biointerfaces 2017 June 2
Physical stability of metal nanoparticle films on planar surfaces can be increased by employing surface modification techniques and/or type of metal nanoparticles. Subsequently, the enzymatic response of colorimetric bioassays can be increased for improved dynamic range for the detection of biomolecules. Using a model bioassay b-BSA, three planar platforms (1) poly (methyl methacrylate) (PMMA) with silver thin films (STFs), (2) silver nanowires (Ag NWs) on paper and (3) indium tin oxide (ITO) on polyethylene terephthalate (PET) were evaluated to investigate the extent of increase in the colorimetric signal. Bioassays for b-BSA and Ki-67 antigen (a real-life bioassay) in buffer were performed using microwave heating (total assay time is 25-30min) and at room temperature (a control experiment, total assay time is 3h). Model bioassays showed that STFs were removed from the surface during washing steps and the extent of ITO remained unchanged. The lowest level of detection (LLOD) for b-BSA bioassays were: 10-10 M for 10nm STFs on PMMA and Ag NWs on paper and 10-11 M for ITO. Bioassays for Ki-67 antigen yielded a LLOD of <10-9 M on ITO platforms, while STFs platforms were deemed unusable due to significant loss of STFs from the surfaces.
Full text links
Related Resources
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app