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JOURNAL ARTICLE
RESEARCH SUPPORT, N.I.H., EXTRAMURAL
RESEARCH SUPPORT, NON-U.S. GOV'T
Derivation of Human-Induced Pluripotent Stem Cells in Chemically Defined Medium.
Human somatic cells can be reprogrammed by defined factors to induced pluripotent stem cells (iPSCs). Importantly, the quality of iPSCs could impact the potential of these cells in basic and clinic research. Here, we describe a method to reprogram human fibroblast cells with Sendai virus in chemically defined conditions, to generate iPSCs that are integration-free and suitable for research and translational applications.
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