Transcriptome-Wide Mapping of N 1 -Methyladenosine Methylome.

N 1 -Methyladenosine (m1 A) is a prevalent posttranscriptional RNA modification and commonly found in tRNA and rRNA. Very recent works have also demonstrated the prevalence of m1 A in mammalian mRNA. Hence, high-throughput methods that allow transcriptome-wide mapping of m1 A will be important for further functional investigations. Here, we describe a technique called "m1 A-ID-Seq", which is based on m1 A immunoprecipitation and the inherent ability of m1 A to stall reverse transcription, to map m1 A in the transcriptome. Utilizing this technique, highly confident m1 A peaks can be obtained on a transcriptome-wide level.