Genome-Wide Location Analyses of N6-Methyladenosine Modifications (m 6 A-Seq).

N6 -methyladenosine-sequencing (m6 A-seq) is a critical tool to obtain an unbiased genome-wide picture of m6 A sites of modification at high resolution. It allows the study of the impact of various perturbations on m6 A modification distribution and the study of m6 A functions. Herein, we describe the m6 A-seq protocol, which entails RNA immunoprecipitation (RIP) performed on fragmented poly(A) RNA utilizing anti-m6 A antibodies. The captured/enriched m6 A positive RNA fragments are subsequently sequenced by RNA-seq in parallel with background control non-immunoprecipitated input RNA fragments. Analyses reveal peaks of m6 A enrichment containing sites of modifications analogous to chromatin modification immunoprecipitation experiments.