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Journal Article
Research Support, Non-U.S. Gov't
Chloroform-free permeabilization for improved detection of β-galactosidase activity in Vibrio cholerae.
Journal of Microbiological Methods 2017 June
LacZ (β-galactosidase) is used to monitor the transcription of genes in reporter strains carrying the lacZ gene under the control of a promotor of interest. This protocol for LacZ activity determinations in Vibrio cholerae following detergent lysis results in 2.5-fold increase of LacZ activities compared to lysis with chloroform.
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