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Two-Color Total Internal Reflection Fluorescence Microscopy of Exocytosis in Endocrine Cells.

Adam J Trexler, Justin W Taraska
Methods in Molecular Biology 2017
We describe a comprehensive method for imaging and analysis of local protein dynamics at single sites of exocytosis in living cultured endocrine cells. This method is well suited to quantitatively map the complex dynamics of individual molecules at single sites of vesicle fusion in live cells.

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