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Cyclic enzymatic repairing-mediated dual-signal amplification for real-time monitoring of thymine DNA glycosylase.
Chemical Communications : Chem Comm 2017 March 31
We develop a new fluorescence method for real-time monitoring of thymine DNA glycosylase (TDG) activity through cyclic enzymatic repairing-mediated dual-signal amplification. This method exhibits excellent sensitivity with a detection limit of 5.6 × 10-7 U μL-1 , and it can be used to determine kinetic parameters and quantify TDG activity from even single cancer cells.
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