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Overnight Enrichment is Essential for Reliable Salmonella PCR Testing from Fecal Samples.
Clinical Laboratory 2017 March 2
BACKGROUND: A rapid molecular test for identifying cases of infectious diarrhea might be beneficial for patients.
METHODS: We established a LightMix modular multiplex PCR assay for rapid detection of Campylobacter, Salmonella, Yersinia, and Shigella species from fecal samples.
RESULTS: Unlike Campylobacter sp., direct detection of Salmonella from feces by multiplex PCR was significantly less sensitive than culture. Only 11 out of 21 (52%) Salmonella culture positive specimens were positive for Salmonella in the direct multiplex PCR assay (Campylobacter: 52/53 samples [98%]). In contrast, an overnight selenite enrichment step prior to multiplex PCR increased the Salmonella assay sensitivity significantly: 18 out of 18 Salmonella culture positive samples were then also detected by PCR.
CONCLUSIONS: An overnight enrichment step is necessary for reliable PCR detection of Salmonella from fecal samples.
METHODS: We established a LightMix modular multiplex PCR assay for rapid detection of Campylobacter, Salmonella, Yersinia, and Shigella species from fecal samples.
RESULTS: Unlike Campylobacter sp., direct detection of Salmonella from feces by multiplex PCR was significantly less sensitive than culture. Only 11 out of 21 (52%) Salmonella culture positive specimens were positive for Salmonella in the direct multiplex PCR assay (Campylobacter: 52/53 samples [98%]). In contrast, an overnight selenite enrichment step prior to multiplex PCR increased the Salmonella assay sensitivity significantly: 18 out of 18 Salmonella culture positive samples were then also detected by PCR.
CONCLUSIONS: An overnight enrichment step is necessary for reliable PCR detection of Salmonella from fecal samples.
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