[Effects of dietary monosodium-glutamate on gene expression].

INTRODUCTION: Nowadays, the food industry more often uses different type of additives during the food production.

AIM: Our aim was to examine the monosodium-glutamate's effect (in animal experiment) on DNA-methyltransferases in gene expression patterns of mRNA levels.

MATERIALS AND METHOD: In the investigation we used 24 (n=24) CD1 type female mice. The animals were fed with different equivalent human doses of the tested substance. After autopsy, mRNA was isolated from different tissues (lung, liver, kidney, spleen). DNMT1, DNMT3A and DNMT3B levels were determined by Quantitative Real-Time PCR.

RESULTS: DNMT1 significantly suppressed the gene expression in all the three treated groups (p<0.05). The DNMT3A expression patterns showed significant decreasing tendency in the 1. and 2. treated groups of the lung tissue (p<0,05) and 1, 2, 3. groups of liver and kidney tissues (p<0,05).

CONCLUSIONS: Our results shows that the monosodium glutamate, suppressed the DNMT1 and DNMT3A gene expression - on mRNA levels of several organs - in mice. It can be a similar chemopreventive effect to epigallo-catechin-gallate's, curcumin's, genistein's, likopine's and rezveratrol's effects. In this case it can be possible that the MSG has anticarcinogenic effects. Orv. Hetil., 2017, 158(10), 380-385.