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Bio-active polymer implants to adipose tissue as in situ source of reprogramming cells.
The implants from natural polymer chitosan was developed by sorption of non-viral DNA vector. These assemblies were shown to be biocompatible and able to transfect the surrounding cells with reprogramming factors. Transgenic rats from the strain allowing visualization of Nanog-positive stem cells received these implants into their adipose deposits. After 8 days we were able to detect in situ-transfected cells. Transfected cells were positive for YFP fluorescence and transiently expressed the markers correlating with stemness.
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