Acquired expression of Cbl Q367P in mice induces dysplastic myelopoiesis mimicking chronic myelomonocytic leukemia.

Chronic myelomonocytic leukemia (CMML) is a hematological malignancy characterized by uncontrolled proliferation of dysplastic myelomonocytes and frequent progression to acute myeloid leukemia (AML). We identified mutations in the Cbl gene, which encodes a negative regulator of cytokine signaling, in a subset of CMML patients. To investigate the contribution of mutant Cbl in CMML pathogenesis, we generated conditional knockin mice for Cbl that express wild-type Cbl in a steady state and inducibly express Cbl Q367P , a CMML-associated Cbl mutant. Cbl Q367P mice exhibited sustained proliferation of myelomonocytes, multilineage dysplasia, and splenomegaly, which are the hallmarks of CMML. The phosphatidylinositol 3-kinase (PI3K)-AKT and JAK-STAT pathways were constitutively activated in Cbl Q367P hematopoietic stem cells, which promoted cell cycle progression and enhanced chemokine-chemokine receptor activity. Gem , a gene encoding a GTPase that is upregulated by Cbl Q367P , enhanced hematopoietic stem cell activity and induced myeloid cell proliferation. In addition, Evi1 , a gene encoding a transcription factor, was found to cooperate with Cbl Q367P and progress CMML to AML. Furthermore, targeted inhibition for the PI3K-AKT and JAK-STAT pathways efficiently suppressed the proliferative activity of Cbl Q367P -bearing CMML cells. Our findings provide insights into the molecular mechanisms underlying mutant Cbl -induced CMML and propose a possible molecular targeting therapy for mutant Cbl -carrying CMML patients.