Cytotoxicity and Antioxidant Potential of Novel 2-(2-((1H-indol-5yl)methylene)-hydrazinyl)-thiazole Derivatives.

Newly synthesized 2-(2-((1 H -indol-5yl)methylene)-hydrazinyl)-thiazole derivatives were evaluated for their in vitro cytotoxicity on two carcinoma cell lines A2780 and HeLa. Significant cytotoxic activity for 2-(2-((1 H -indol-5-yl)methylene)hydrazinyl)-4-methylthiazole ( 1 ) and 2-(2-((1 H -indol-5-yl)methylene)hydrazinyl)-4-phenylthiazole ( 3 ), on both A2780 [IC50 : 11.6 μM ( 1 ), and 12.4 μM ( 3 )] and HeLa [IC50 : 22.4 μM ( 1 ) and 19.4μM ( 3 )] cell lines is reported. Their antioxidant potential was evaluated by spectrophotometric method, using DPPH radical or Fe (TPTZ)3+ complex, and EPR spectroscopy, therefore the compounds 1 and 3 showed remarkable antioxidant activity simultaneously with a cytotoxic effect on A2780 and HeLa cell lines. Furthermore, based on theoretical quantum chemical calculation, the present study analyzed the chemoselectivity of the hydrogen extraction from the indolyl-hydrazinil-thiazoles in reaction with free radicals.