Characterization of the pJB12 Plasmid from Pseudomonas aeruginosa Reveals Tn 6352 , a Novel Putative Transposon Associated with Mobilization of the bla VIM-2 -Harboring In58 Integron.

The bla VIM-2 -carrying In58 integron has been linked to a chromosomal location in different bacterial species, including Pseudomonas aeruginosa This work reports the first fully sequenced In58-harboring plasmid, which is significantly different from the two previously identified bla VIM-2 -carrying plasmids in P. aeruginosa bla VIM-2 might have been acquired by transposition of Tn 6352 , a novel transposon composed of the In58 and IS Pa17 elements. The recognition of similar inverted repeat (IR) sites by IS Pa17 reveals a common mobilization process associated with acquisition of the bla VIM-2 and bla VIM-1 genes.