Preparation of Arjunāriṣṭa Using Microbes Isolated from Woodfordia fruticosa Flowers (Dhayati).

OBJECTIVE: To verify the utility of isolated fermentative microbes from Woodfordia fruticosa flowers for preparation of Arjunāriṣṭa formulation and its comparative evaluation with the same formulation prepared by traditional method.

METHODOLOGY: In the present technique, isolated colonies of microorganisms from Woodfordia fruticosa (Dhātakī) flowers on Saubroad dextrose media were separated and suspended in sterile water. This suspension was aseptically added in previously sterilized mixtures containing all intended ingredients for Arjunāriṣṭa which was thereafter incubated for 20 days at 37°C to achieve optimal fermentation. The formulation thus obtained was further subjected to various evaluation tests.

RESULT: Arjunāriṣṭa was prepared using a new approach, and in that, isolated microorganisms from the flowers of Woodfordia fruticosa (Dhātakī) were used. It was found that the new approach was successful in generating approximately same quantities of alcohol content in comparison with traditional methods which have shown varying concentration of alcoholic content. Moreover, the new process prevents the growth of unwanted microbes thus, optimizing standards for purity and safety of the formulation.

CONCLUSION: The formulation prepared by a new procedure showed marked uniformity for different parameters such as alcohol production, total phenol content, total solid content as compared to that prepared by the traditional method. Similarly, the results of thin layer chromatography, high performance thin layer chromatography showed marked uniformity related to quality, safety, efficacy, and reproducibility of the new method as compared to the traditional one. Thus, the modern technique was found to show reproducibility and facilitate easier quality assessment.