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Research Support, Non-U.S. Gov't
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Rapid diagnosis of Vibrio owensii responsible for shrimp acute hepatopancreatic necrosis disease with isothermal recombinase polymerase amplification assay.

Liyuan Liu, Luzhi Jiang, Yongxin Yu, Xiaoming Xia, Yingjie Pan, Shuling Yan, Yongjie Wang
Molecular and Cellular Probes 2017 June
A rapid and sensitive AHPND-RPA assay was developed for the specific detection of the AHPND-causing Vibrio owensii. The AHPND-RPA detected as few as 2 copies per reaction in 9.02 ± 0.66 min at 39 °C, and showed 100% positive predictive value and negative predictive value for AHPND diagnosis.

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