Highly Selective and Repeatable Surface-Enhanced Resonance Raman Scattering Detection for Epinephrine in Serum Based on Interface Self-Assembled 2D Nanoparticles Arrays.

Target analyte detection in complex systems with high selectivity and repeatability is crucial to analytical technology and science. Here we present a two-dimensional (2D) surface-enhanced resonance Raman scattering (SERRS) platform, which takes advantages of the high selectivity of the SERRS sensor as well as the sensitivity and reproducibility of the interfacial SERS platform, for detecting trace epinephrine (EP) in the serum. To realize sensitive and selective detection of EP in a complex system, Au NPs are modified with α,β-nitriloacetic acid and Fe(NO3 )3 to form the Au NP-(Fe-NTA) sensor, and as a consequence, EP can be rapidly captured by the sensor on the surface of Au NPs and then delivered at the cyclohexane/water interface. More importantly, we synthesized the extremely stable Au NPs (PVP-stabilized Au NPs), where the presence of PVP prevents aggregation of Au NPs during the self-assembly process and then makes a more uniform distribution of Au NPs with analytes at the cyclohexane/water interface, approximately 2 nm interparticle distance between the Au NPs, which has been proved by synchrotron radiation grazing incidence small-angle X-ray scattering (SR-GISAXS) experiments. The self-assembly method not only effectively avoids the aggregation of Au NPs and decreases the influence of the background signal but also can capture and enrich EP molecules in the cyclohexane/water interface, realizing the sensitive and selective detection of EP in complex serum sample. This strategy overcomes the difficulty of bringing nanostructures together to form efficient interparticle distance with simple fabrication and maximum uniformity and also provides a powerful nanosensor for tracing amounts of analyte molecules in a complex system with the advantages of capturing and enriching of target molecules in the liquid/liquid interface during the self-assembly process. Our SERRS platform opens vast possibilities for repeatability, sensitivity, and selectivity detection of targets in various complex fields.