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Double-Immunodiffusion Assay for Detecting Specific Antibodies (Ouchterlony).
Current Protocols in Immunology 2017 Februrary 3
The method first described by Ouchterlony in 1948 is a classic and simple technique that permits evaluation and comparison of antibodies in animal or human sera directed against protein or complex carbohydrate antigens. It is a low-tech procedure that may provide information on the relative quantity of antibody activity and the nature of the antigenic epitopes in different preparations. © 2017 by John Wiley & Sons, Inc.
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