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One-pot native chemical ligation by combination of two orthogonal thioester precursors.

Yuya Asahina, Toru Kawakami, Hironobu Hojo
Chemical Communications : Chem Comm 2017 Februrary 10
We developed a one-pot peptide ligation method using two orthogonal thioester precursors and a protecting group for the ligation reaction between Asp and Cys. Combination of the two precursors facilitated the one-pot operation and yielded the entire polypeptide. The usefulness of this method was successfully demonstrated by the total synthesis of histone H4.

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