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Subharmonic Response of Polymer Contrast Agents Based on the Empirical Mode Decomposition.

The subharmonic threshold for ultrasound contrast agent has been defined as a 20-25 dB difference between fundamental and subharmonic (2/1) spectral components of the backscatter signal. However, this Fourier-based criteria assumes a linear, time-invariant signal. A more appropriate criterion for short cycle and frequency modulated waveforms is proposed with an adaptive signal-processing approach based on the Empirical Mode Decomposition (EMD) method. The signal is decomposed into an orthogonal basis known as Intrinsic Mode Functions (IMFs) and a subharmonic threshold is defined with respect to the energy ratio of the subharmonic IMF component to that of the incident signal. The method is applied to backscatter data acquired from two polymer-shelled contrast agents (Philips #38, mean diameter 2.0 m) and Point Biomedical (#12027, mean diameter 3.9 m). The acoustic backscatter signals are investigated for a single contrast agent subjected to monofrequency (20 MHz, 20 cycles) and chirp (15-25 MHz, 20 cycles) forcing for incident pressures ranging from 0.5 to 2.4 MPa. In comparison to the spectral peak difference (20 dB) criterion, the EMD method is more sensitive in determining subharmonic signals.

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