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JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
Probing transport of charged β-lactamase inhibitors through OmpC, a membrane channel from E. coli.
Biochemical and Biophysical Research Communications 2017 Februrary 27
One of the major causes of antibiotic resistance in the Gram-negative bacteria is the low permeability across the outer membrane. Currently a main bottleneck in the development of effective antibiotics is the lack of a general method to quantify permeation which would allow screening for optimal scaffolds. Here, we present a permeation assay based on conventional electrophysiology. The method mainly involves application of concentration gradients of charged molecules with different electrophoretic mobilities through a membrane channel. Thus the unbalanced flux creates an electrostatic potential which provides direct information on relative ion fluxes. The experimental approach applied here involves measuring zero-current-potentials and the corresponding single channel conductance. For OmpC and the β-lactamase inhibitor avibactam at a 10 μm gradient the calculated flux rate at Vm =0mV was about n=200 molecules/s per OmpC single pore.
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