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JOURNAL ARTICLE
RESEARCH SUPPORT, NON-U.S. GOV'T
Photoinduced Cross-Linking of Short Furan-Modified DNA on Surfaces.
Langmuir : the ACS Journal of Surfaces and Colloids 2017 Februrary 8
We report for the first time the formation of site-specific interstrand cross-linked (ICL) surface-immobilized furan-modified DNA duplexes via singlet oxygen. 1 O2 , necessary for effecting furan-mediated ICL formation, was produced in situ using methylene blue or a zinc phthalocyanine derivative (TT1) as a photosensitizer. Via surface plasmon resonance spectroscopy, we show that surface ICL was achieved, and a robust link formed that enhances the stability of the 12-mer duplex even after surface regeneration. The described method represents a novel platform technology based on surfaces with addressable and stable DNA duplexes requiring only short oligonucleotides.
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