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Perfect 1 J CH -resolved HSQC: Efficient measurement of one-bond proton-carbon coupling constants along the indirect dimension.

N Marcó, A A Souza, P Nolis, R R Gil, T Parella
Journal of Magnetic Resonance 2017 March
A versatile 1 JCH -resolved HSQC pulse scheme for the speedy, accurate and automated determination of one-bond proton-carbon coupling constants is reported. The implementation of a perfectBIRD element allows a straightforward measurement from the clean doublets obtained along the highly resolved F1 dimension, even for each individual 1 JCHa and 1 JCHb in diastereotopic Ha CHb methylene groups. Real-time homodecoupling during acquisition and other alternatives to minimize accidental signal overlapping in overcrowded spectra are also discussed.

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