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Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Expression and Purification of SH2 Domains Using Baculovirus Expression System.
Recombinant proteins expressed in bacteria are sometimes insoluble, aggregated, and incorrectly folded. For those Src homology 2 (SH2) domains that are insoluble in bacteria, baculovirus-insect cell expression systems can be an alternative to produce soluble and functionally active proteins. We describe a protocol for cloning and purification of GST-tagged SH2 domains using the Bac-to-Bac baculovirus expression system.
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