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Development of simple and sensitive hydrogel based colorimetric sensor array for the real-time quantification of gaseous ammonia.
A real-time colorimetric sensor array (CSA) offers the advantages of diversity and accuracy for the quantification of multiple analytes; however, traditional sensors require a complex fabrication process. Therefore, to take full advantage of this sensing platform, we have developed a simple CSA system composed of a polymer, a reducing agent, and different pH indicators. Distinctive color response patterns were classified by extracting the hidden information, (i.e., red, green, and blue (RGB) values) from the indicators. This triple-channel sensing platform is further applied for statistical analysis, to quantify different concentrations of ammonia and other analytes. The sensor array showed a limit of detection of 0.3ppm, which is well below the diagnostic criteria for ammonia concentration in the breath of healthy individuals and of patients with end-stage renal disease. As this sensor would be able to quantify gaseous ammonia in the breath, it is relevant to the point-of-care diagnosis of patients with renal diseases.
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