Differences in the expression of cell cycle genes in osteoblasts and endothelial cells cultured on the surfaces of Ti6Al4V and Ti6Al7Nb alloys.

Three medically used alloys (Ti6Al4V, Ti6Al7Nb, and AISI 316 L) are compared due to proliferative potential and metabolic response of human cells (osteoblasts line Saos-2 and endothelial cells line EA.hy-926) seeded on the surfaces of these alloys. Although no statistically significant difference in the proliferative potential of the cells cultured on the surfaces of examined biomaterials was observed, it does not exclude relevant differences in metabolic response of these cells assessed as changes in genes' expression. As a result of our studies it was demonstrated that the changes in the expression of examined genes were very common. Our observation suggests the presence of the process of selective recognition of the contacted biomaterials by the cells seeded on their surfaces. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1607-1617, 2017.