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Evaluation of a commercial loop-mediated isothermal amplification assay for diagnosis of Bordetella pertussis infection.

Kazunari Kamachi, Takumi Moriuchi, Yukihiro Hiramatsu, Nao Otsuka, Keigo Shibayama
Journal of Microbiological Methods 2017 Februrary
We evaluated a commercial loop-mediated isothermal amplification (LAMP) assay kit for Bordetella pertussis detection. The LAMP primers were designed to target the ptxP1 allele of the pertussis toxin promoter, but the assay could detect B. pertussis ptxP3 and ptxP8 strains in addition to ptxP1 strains, with high analytical sensitivity.

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