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IFN- promoter polymorphisms do not affect QuantiFERON TB Gold In-Tube test results in a Canadian population.

BACKGROUND: Several studies have shown polymorphisms within the interferon-gamma (IFN-) promoter influence cytokine expression. The interferon-gamma release assay (IGRA) relies on the ability to produce IFN- in response to tuberculosis (TB) specific antigens. This study determined the relationship between the IFN- +874 A/T promoter polymorphism and the performance of the QuantiFERON-TB Gold In-Tube (QFT-GIT) test in an ethnically diverse Canadian population.

METHODS: A total of 190 participants were categorised into three groups based on history of and exposure to TB: active TB (n = 55), TB exposed (n = 55) and presumably TB unexposed controls (n = 80). All participants underwent QFT-GIT testing, and DNA was extracted from whole blood and probed for polymorphism at position +874 (T/A) of intron 1 of IFN-. Statistical relationships between the QFT-GIT results, polymorphisms and demographic data were evaluated.

RESULTS: IFN- +874 genotype frequencies among the entire study population (n = 190) were A/A (45.8%), T/A (39.5%), and T/T (14.7%). Among the three study groups, there was no correlation between QFT-GIT results and the IFN- +874 A/T genotype, and no correlation of genotype with IFN- production in response to either Mycobacterium tuberculosis antigens or mitogenic stimulation.

CONCLUSION: Our results indicate that the IFN- +874 promoter polymorphism does not influence QFT-GIT performance in this study population.

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