We have located links that may give you full text access.
Enriching s 4 U-RNA Using Methane Thiosulfonate (MTS) Chemistry.
Current Protocols in Chemical Biology 2016 December 8
Metabolic labeling of cellular RNA is a useful approach to study RNA biology. 4-Thiouridine (s4 U) is a convenient nucleoside for metabolic labeling because it is cell permeable and is incorporated into newly transcribed RNA, and the sulfur moiety provides a handle for biochemical purification. However, a critical step in the purification of s4 U-RNA is the efficiency of the chemistry used to enrich s4 U-RNA. Here, we present a protocol for s4 U-RNA enrichment that includes efficient and reversible covalent chemistry to biotinylate s4 U-RNA using the activated disulfide methane thiosulfonate conjugated to biotin (MTS-biotin), followed by enrichment on streptavidin beads. The efficiency of this chemistry reduces enrichment bias and requires less starting material, thereby expanding the utility of s4 U to study RNA biology. © 2016 by John Wiley & Sons, Inc.
Full text links
Related Resources
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app