Preservation of Malassezia pachydermatis using skimmed milk as cryoprotective agent.

F A Silva Sergent, B Acosta-Hernández, J Raduán Jaber, I Rosario Medina, O Ferrer Quintana, F Real Valcárcel, D Padilla Castillo, S Déniz Suárez

Journal of Microbiological Methods 2017 January

This study evaluated the effect of skimmed milk as a cryoprotectant for the maintenance and long-term preservation of 70 Malassezia pachydermatis strains. An initial suspension of each strain was prepared in sterile distilled water with skimmed milk. The most effective method was cryopreservation that ensured 100% viability for 2years.

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