Add like
Add dislike
Add to saved papers

Characterization of cis-elements in the Promoter of trz2 Encoding Schizosaccharomyces pombe Mitochondrial tRNA 3'-end Processing Enzyme.

Microbiology 2016 November 23
The endonuclease tRNase Z is responsible for the 3'-end processing of tRNA precursors, which is one of the essential steps in tRNA maturation. The fission yeast Schizosaccharomyces pombe contains two essential tRNase ZL genes (trz1 and trz2) involved in nuclear and mitochondrial tRNA 3'-end processing, respectively. Our previous studies suggest that trz2 is expressed at a very low level. Here we report characterization of the trz2 promoter. Using lacZ as a reporter, we show that the trz2 promoter contains a HomolD box and a very weak diverged TATA element. The HomolD box is usually found in the promoters of S. pombe ribosomal protein genes. lacZ reporter assays suggest that the HomolD box regulates the expression of both trz2 and the ribosomal protein gene rps2501, which are arranged head-to-head on opposite strands. Overexpression of Rrn7, a candidate HomolD box binding protein, up-regulates expression of lacZ under the control of the trz2 promoter or the rps2501 promoter. Functional complementation studies suggest that the TATA-like element is essential for trz2 expression, whereas the HomolD box may play a nonessential regulatory role. We also demonstrate that a 57-nt negative regulatory region (NRE) located between the HomolD box and the TATA-like element represses the expression of lacZ under the control the trz2 promoter. Our results suggest that the low-level trz2 expression may arise from a low level of transcription caused by lack of a strong TATA box and the NRE. Our analysis also suggests that trz2 and rps2501 may be coregulated by the HomolD box.

Full text links

We have located links that may give you full text access.
Can't access the paper?
Try logging in through your university/institutional subscription. For a smoother one-click institutional access experience, please use our mobile app.

For the best experience, use the Read mobile app

Mobile app image

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app

All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.

By using this service, you agree to our terms of use and privacy policy.

Your Privacy Choices Toggle icon

You can now claim free CME credits for this literature searchClaim now

Get seemless 1-tap access through your institution/university

For the best experience, use the Read mobile app